PDF DatasheetRat CNTF Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CNTF based upon sequence from Rat
Host: QP11473 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CNTF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11473.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CNTF protein solution (0.5 mg/ml) containing phosphate buffered saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Rat CNTF General Information | |
|---|---|
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| Alternate Names | |
| Ciliary neurotrophic factor, CNTF, Cntf. | |
| Molecular Weight | |
| 25.2 kDa | |
| Chromosomal Location | |
| G3 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Cst3 |
| Entrez Gene ID | 13010 |
| UniProt ID(s) | P21460 |
| COSMIC ID Link(s) | Cst3 |
| KEGG Gene ID(s) | mmu:13010 |
| General Description of Recombinant Rat CNTF. | |
| Rat CNTF produced in E. coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-200AA) and having a molecular mass of 25.2kDa. CNTF is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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