PDF DatasheetHuman HSP27 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HSP27 based upon sequence from Human
Host: QP12327 protein expressed in E. Coli.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of HSP27 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12327.
Bioactivity Data: Untested
Amino Acid Sequence: MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HSP27 protein solution contains 20mM Hepes pH 7.5, 1mM DTT and 100mM KCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human HSP27 General Information | |
|---|---|
| Alternate Names | |
| HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS. 76067, DKFZp586P1322. | |
| Molecular Weight | |
| 22.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P04792 |
| General Description of Recombinant Human HSP27. | |
| Recombinant Human HSP-27 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids and having a molecular mass of 22.7 kDa. Hsp27 was over-expressed in E. coli and purified by conventional chromatography. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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