PDF DatasheetHuman S100B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant S100B based upon sequence from Human
Host: QP13404 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of S100B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP13404.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: S100B (1 mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human S100B General Information | |
|---|---|
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| Alternate Names | |
| Protein S100-B, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, S100B, NEF, S100, S100beta. | |
| Molecular Weight | |
| 12.8 kDa | |
| Chromosomal Location | |
| q22.3 on chromosome 21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | S100B |
| Entrez Gene ID | 6285 |
| UniProt ID(s) | P04271 |
| COSMIC ID Link(s) | S100B |
| KEGG Gene ID(s) | hsa:6285 |
| General Description of Recombinant Human S100B. | |
| Human S100B produced in E. Coli is single, a non-glycosylated, Polypeptide chain containing 112 amino acids fragment (1-92) with a 20 amino acids N-terminal His tag and having a total molecular mass of 12.8kDa. The S100B is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
Oxidative stress-induced S100B proteinfrom placenta and amnion affects soluble Endoglin release from endothelialcellsPublication: Molecularhuman reproduction 16.3 (2010): 188-199.Link: http://molehr.oxfordjournals.org/content/16/3/188.full
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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