PDF DatasheetMouse GDF5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GDF5 based upon sequence from Mouse
Host: QP6075 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) GDF5, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6075.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala376 – Arg495
Amino Acid Sequence: APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Mouse GDF5 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse GDF5 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Gdf5 |
| Entrez Gene ID | 14563 |
| Ensemble Gene ID | ENSMUSG00000038259 |
| RefSeq Protein Accession(s) | NP_032135.2 |
| RefSeq mRNA Accession(s) | NM_008109.3 |
| UniProt ID(s) | P43027 |
| UniGene ID(s) | Mm.4744 |
| COSMIC ID Link(s) | Gdf5 |
| KEGG Gene ID(s) | mmu:14563 |
| General Description of Recombinant Mouse GDF5 Protein. | |
| Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction . Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG . Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 . Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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