PDF DatasheetHuman PCNA Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PCNA based upon sequence from Human
Host: QP6470 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) PCNA, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6470.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ser261
Amino Acid Sequence: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human PCNA Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human PCNA Protein General Information | |
|---|---|
| Alternate Names | |
| PCNA, ATLD2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PCNA |
| Entrez Gene ID | 5111 |
| Ensemble Gene ID | ENSG00000132646 |
| RefSeq Protein Accession(s) | NP_002583.1 |
| RefSeq mRNA Accession(s) | NM_002592.2, NM_182649.1 |
| UniProt ID(s) | P12004 |
| UniGene ID(s) | Hs.147433 |
| HGNC ID(s) | HGNC:8729 |
| COSMIC ID Link(s) | PCNA |
| KEGG Gene ID(s) | hsa:5111 |
| PharmGKB ID(s) | PA263 |
| General Description of Recombinant Human PCNA Protein. | |
| Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while ‘Lys-63’-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
![Analysis of Mass Spec data (dashed-line) of fractions stained with PCNA MS-QAVA™ monoclonal antibody [Clone: PC10] (solid-line), reveals that less than 3.7% of signal is attributable to non-specific binding of anti-PCNA [Clone PC10] to targets other than PCNA protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-5111-MSM1-P1-anti-PCNA-antibody-150x150.png)
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