PDF DatasheetE. coli Elongation factor P Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Elongation factor P based upon sequence from: E. coli
Host: QP7316 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Elongation factor P, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7316.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Lys188
Amino Acid Sequence: ATYYSNDFRA GLKIMLDGEP YAVEASEFVK PGKGQAFARV KLRRLLTGTR VEKTFKSTDS AEGADVVDMN LTYLYNDGEF WHFMNNETFE QLSADAKAIG DNAKWLLDQA ECIVTLWNGQ PISVTPPNFV ELEIVDTDPG LKGDTAGTGG KPATLSTGAV VKVPLFVQIG EVIKVDTRSG EYVSRVK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Elongation factor P Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Elongation factor P Protein General Information | |
|---|---|
| Alternate Names | |
| ECK4141; JW4107; b4147 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | efp |
| Entrez Gene ID | 948661 |
| RefSeq Protein Accession(s) | NP_418571.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A6N4 |
| KEGG Gene ID(s) | ecj:JW4107 |
| General Description of Recombinant E. coli Elongation factor P Protein. | |
| Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Beta-lysylation at Lys-34 is required for alleviation. The Pro codons and their context do not affect activity; only consecutive Pro residues (not another amino acid) are affected by EF-P. Has stimulatory effects on peptide bond formation between ribosome-bound initiator tRNA(fMet) and puromycin, and N-acetyl-Phe tRNA(Phe)-primed poly(U)-directed poly(Phe) synthesis. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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