PDF DatasheetE. coli Elongation factor P Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Elongation factor P based upon sequence from: E. coli
Host: QP9742 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Elongation factor P, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9742.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Lys188
Amino Acid Sequence: MATYYSNDFR AGLKIMLDGE PYAVEASEFV KPGKGQAFAR VKLRRLLTGT RVEKTFKSTD SAEGADVVDM NLTYLYNDGE FWHFMNNETF EQLSADAKAI GDNAKWLLDQ AECIVTLWNG QPISVTPPNF VELEIVDTDP GLKGDTAGTG GKPATLSTGA VVKVPLFVQI GEVIKVDTRS GEYVSRVK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Elongation factor P Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Elongation factor P Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | efp |
| RefSeq Protein Accession(s) | WP_000257278.1 |
| RefSeq mRNA Accession(s) | NC_010473.1 |
| UniProt ID(s) | B1XDQ0 |
| KEGG Gene ID(s) | ecd:ECDH10B_4340 |
| General Description of Recombinant E. coli Elongation factor P Protein. | |
| Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Modification of Lys-34 is required for alleviation. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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