PDF DatasheetM. tuberculosis Ag85A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Ag85A based upon sequence from: M. tuberculosis
Host: QP8929 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) Ag85A, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8929.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Tyr53 – Thr331
Amino Acid Sequence: YLQVPSPSMG RDIKVQFQSG GANSPALYLL DGLRAQDDFS GWDINTPAFE WYDQSGLSVV MPVGGQSSFY SDWYQPACGK AGCQTYKWET FLTSELPGWL QANRHVKPTG SAVVGLSMAA SSALTLAIYH PQQFVYAGAM SGLLDPSQAM GPTLIGLAMG DAGGYKASDM WGPKEDPAWQ RNDPLLNVGK LIANNTRVWV YCGNGKPSDL GGNNLPAKFL EGFVRTSNIK FQDAYNAGGG HNGVFDFPDS GTHSWEYWGA QLNAMKPDLQ RALGATPNT
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of M. tuberculosis Ag85A Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant M. tuberculosis Ag85A Protein General Information | |
|---|---|
| Alternate Names | |
| ; Diacylglycerol acyltransferase/mycolyltransferase Ag8 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | fbpA |
| RefSeq Protein Accession(s) | WP_003900759.1 |
| RefSeq mRNA Accession(s) | NZ_KK341227.1 |
| UniProt ID(s) | P9WQP2 |
| KEGG Gene ID(s) | mtc:MT3911 |
| General Description of Recombinant M. tuberculosis Ag85A Protein. | |
| The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor (By similarity). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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