PDF DatasheetHuman HMGB1 / HMG1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HMGB1 / HMG1 based upon sequence from: Human
Host: QP9179 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) HMGB1 / HMG1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9179.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Gly2 – Glu215
Amino Acid Sequence: GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGETKKKFKD PNAPKRPPSA FFLFCSEYRP KIKGEHPGLS IGDVAKKLGE MWNNTAADDK QPYEKKAAKL KEKYEKDIAA YRAKGKPDAA KKGVVKAEKS KKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human HMGB1 / HMG1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human HMGB1 / HMG1 Protein General Information | |
|---|---|
| Alternate Names | |
| HMGB1; HMG1; HMG3; SBP-1; HMG-1; HMG1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Hmgb1 |
| Entrez Gene ID | 3146 |
| Ensemble Gene ID | ENSG00000189403 |
| RefSeq Protein Accession(s) | NP_001300821.1 |
| RefSeq mRNA Accession(s) | NM_001313892.1, NM_001313893.1, NM_002128.5 |
| UniProt ID(s) | P09429 |
| UniGene ID(s) | Hs.434102 |
| HGNC ID(s) | HGNC:4983 |
| COSMIC ID Link(s) | HMGB1 |
| KEGG Gene ID(s) | hsa:3146 |
| PharmGKB ID(s) | PA188 |
| General Description of Recombinant Human HMGB1 / HMG1 Protein. | |
| Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the Extracellular domain environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the Extracellular domain compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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