PDF DatasheetRat Tnf alpha Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Tnf alpha based upon sequence from Rat
Host: QP5508 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5508.
Bioactivity Data: Cytolysis of mouse L929 cells in the presence of Actinomycin D significantly induced by less than 50 pg/ml corresponding to an activity of at least 2.0 x 10^7 units/mg (or a typical ED50 of less than 10 pg/ml).
Monomer or Dimer: Monomer
Amino Acid Sequence: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: A Certificate of Analysis (CofA) will be sent along with the protein and will contain the proper reconstitution instructions.
Concentration of Rat Tnf alpha Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Rat Tnf alpha Protein General Information | |
|---|---|
| Alternate Names | |
| Tnfa, RATTNF, TNF-alpha | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tnf |
| Entrez Gene ID | 103694380 |
| Ensemble Gene ID | ENSRNOG00000000837 |
| RefSeq Protein Accession(s) | NP_036807.1 |
| RefSeq mRNA Accession(s) | NM_012675.3, XM_008772775.2 |
| UniProt ID(s) | P16599 |
| UniGene ID(s) | Rn.2275 |
| COSMIC ID Link(s) | Tnf |
| KEGG Gene ID(s) | rno:103694380 |
| General Description of Recombinant Rat Tnf alpha Protein . | |
| Tumor necrosis factor alpha (TNFa) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF? signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF? functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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