Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-PSAP (clone rAIF1/1909) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-PSAP antibody (Clone rAIF1/1909). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Analysis of Protein Array containing >19,000 full-length human proteins using AIF1 Mouse Monoclonal Antibody (rAIF/1909)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 228.00$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 199-MSM2
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
199-MSM2-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
199-MSM2-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
199-MSM2-P1ABX Size: 100 ug, Tag: Unconjugated $ 488.00
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SKU: 199-MSM2-P0
AIF1 / Iba1 (Microglia Marker) General Information
Alternate Names
Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)
Molecular Weight
17kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol199
Entrez Gene IDAIF1
UniProt ID(s)P55008
UniGene ID(s)Hs76364
COSMIC ID Link(s)AIF1
KEGG Gene ID(s)hsa:199
General Description of AIF1 / Iba1 (Microglia Marker).
AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Human Anti-AIF1 / Iba1 (Microglia Marker) Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-AIF1 / Iba1 (Microglia Marker) Antibody Clone: rAIF1/1909
Clone rAIF1/1909 Host and Isotype: Mouse IgG1
Anti-Human AIF1 / Iba1 (Microglia Marker) Positive Control Sample: Lymph Node, Tonsil or Kidney
Cellular Localization of Antibody rAIF1/1909 Staining: Cytoplasmic and Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Purified recombinant human AIF1 protein (apprx. aa 1-146) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

AIF1 / Iba1 (Microglia Marker) Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's product, AIF1 / Iba1 (Microglia Marker) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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AIF1 / Iba1 (Microglia Marker) Ultraspecific Antibody

Tested against >20,000 Human Proteins

https://enquirebio.com/wp-content/uploads/2019/01/Anti-PSAP Specificity Assay (Array)-300x120.jpghttps://enquirebio.com/wp-content/uploads/2019/01/PSAP IHC Human Tonsil (rAIF1 1909)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Anti-PSAP(rAIF1 1909) Gel-245x300.jpghttps://enquirebio.com/wp-content/uploads/2018/06/enQuire-Bio-199-MSM2-P0-anti-AIF1-Iba1-Microglia-Marker-antibody-1-300x120.jpeg