Human Anti-Caldesmon, HMW Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Anti-Caldesmon, HMW Antibody Clone: CALD1/820 + h-CALD
Clone CALD1/820 + h-CALD Host and Isotype: Mouse IgG
Anti-Human Caldesmon, HMW Positive Control Sample: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CALD1 protein (CALD1/820); Crude human uterus extract (h-CALD)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Caldesmon, HMW Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CALD1 antibody staining is expected to be primarily localized to the actin filaments and plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in Caldesmon, HMW antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, decidual cells in the placenta, ovarian stroma cells in the ovary and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, decidual cells in the placenta, ovarian stroma cells in the ovary and smooth muscle cells in the smooth muscle. Low, but measureable presence of Caldesmon, HMW could be seen inadipocytes in breast, cells in the seminiferous ducts in testis, cells in the tubules in kidney, fibroblasts in mesenchymal tissue, glandular cells in the breast, endometrium, epididymis, prostate, salivary gland and stomach, hepatocytes in liver, islets of Langerhans in pancreas, Leydig cells in the testis, macrophages in lung, pneumocytes in lung, squamous epithelial cells in the cervix and uterine, oral mucosa and tonsil. We were unable to detect Caldesmon, HMW in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Caldesmon, HMW Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.