Mouse Anti-CD28 Antibody Product Attributes
Tested Applications: Flow Cytometry, immunoprecipitation.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD28 Antibody Clone: 37.51
Clone 37.51 Host and Isotype: Golden Syrian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD28 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD28 / TP44 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD28 / TP44 could be seen in cells in the red pulp in spleen, germinal center cells in the tonsil, hematopoietic cells in the bone marrow and lymphoid tissue in appendix. We were unable to detect CD28 / TP44 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Johnston RJ, Choi YS, Diamond JA, Yang JA, and Crotty S. 2012. J. Exp. Med. 209:243-250. (in vitro activation)Hafalla JCR, Burgold J, Dorhoi A, Gross O, Ruland J, Kaufmann SHE, and Matuschewski K. 2012. Infect. Immun. 80:1274-1279. (in vitro activation)Driessens G, Zheng Y, Locke F, Cannon JL, Gounari F, and Gajewski TF. 2011. J. Immunol. 186:784-790. (Flow cytometry)Alcazar I, Cortes I, Zaballos A, Hernandez C, Fruman DA, Barber DF, and Carrera AC. 2009. Blood. 113:3198-3208. (Immunoprecipitation, in vitro activationAlbert MH, Yu X-Z, Martin PJ, and Anasetti C. 2005. Blood. 105:1355-1361. (in vivo activation)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD28 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.