Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: Hit3a
Clone Hit3a Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD3 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3 could be seen in. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Lesourne R, Zvezdova E, Song K-D, El-Khoury D, Uehara S, Barr VA, Samelson LE and Love PE. 2012. J. Immunol. 189: 1154-1161. (in vitro activation)Knyazhitsky M, Moas E, Shaginov E, Luria A, and Braiman A. 2012. J. Biol. Chem. 287: 19725-19735. (in vitro activation)Ge Shuwang, Hertel B, Emden SH, Beneke J, Menne J, Haller H, and von Vietinghoff S. 2012. Nephrol. Dial. Transplant. 27: 2768-2772. (Immunofluorescence microscopy)Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM, and Varki A. 2010. J. Immunol. 184: 4185-4195. (Flow cytometry - Chimpanzee)Westermann J, Bode U, Sahle A, Speck U, Karin N, Bell EB, Kalies K, and Gebert A. 2005. J. Immunol. 174: 2517-2524. (Immunohistochemistry - frozen tissue)Mukouyama H, Janzen NK, Hernandez JM, Lam JS, Caliliw R, Wang AY, Figlin RA, Belldegrun AS, and Zeng G. 2004. Clin. Cancer Res. 10: 1421-1429. (in vitro blocking)
Limitations and Warranty
enQuire Bio's Human Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.