Human peripheral blood monocytes were stained with 5 uL  (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human peripheral blood monocytes were stained with 5 uL  (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human peripheral blood monocytes were stained with 5 uL  (solid line) or 0.5 ug PE Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD54 / ICAM-1 Antibody [15.2]

$ 88.00$ 328.00
Please Select Product Options Below To View The Catalog Number.

Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;PE
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB47-F-25Tests Size: 25 tests, Tag: FITC $ 79.00
QAB47-100ug Size: 100 ug, Tag: Unconjugated $ 99.00
QAB47-APC-100Tests Size: 100 tests, Tag: APC $ 289.00
QAB47-APC-25Tests Size: 25 tests, Tag: APC $ 109.00
QAB47-F-100Tests Size: 100 tests, Tag: FITC $ 199.00
QAB47-PE-100Tests Size: 100 tests, Tag: PE $ 219.00
QAB47-PE-25Tests Size: 25 tests, Tag: PE $ 89.00
QAB47-QF630-100ug Size: 100 tests, Tag: Qfluor™ 630 $ 179.00
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Datasheets and Documentation
Product Datasheet
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SKU: QAB47-F-25Tests
CD54 / ICAM-1 General Information
Alternate Names
Curated Database and Bioinformatic Data
Gene SymbolICAM1
Entrez Gene ID3383
Ensemble Gene IDENSG00000090339
RefSeq Protein Accession(s)NP_000192
RefSeq mRNA Accession(s)NM_000201
RefSeq Genomic Accession(s)NC_000019, NG_012083, NC_018930
UniProt ID(s)P05362
UniGene ID(s)P05362
HGNC ID(s)5344
Cosmic ID(s)ICAM1
KEGG Gene ID(s)hsa:3383
PharmGKB ID(s)PA29592
General Description of CD54 / ICAM-1.
The 15.2 antibody reacts with human CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1), a 90-110 kDa cell surface glycoprotein that is inducibly expressed on both immune and endothelial cells. As its name implies, ICAM-1 participates in cell-cell adhesion between leukocytes and endothelial cells, facilitating leukocyte recruitment and transmigration at sites of inflammation. The ligands for ICAM-1 are also expressed on leukocyte and endothelial cells, and include Mac-1, fibrinogen, and a member of the integrin protein family, LFA-1 (CD11a).The 15.2 antibody may be used for analysis of ICAM-1 expression in human cells and tissues, and is reported to be cross-reactive with porcine ICAM-1.

Human Anti-CD54 / ICAM-1 Antibody Product Attributes

Species: Human
Cross Reactivity: pig
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD54 / ICAM-1 Antibody Clone: 15.2
Clone 15.2 Host and Isotype: Mouse IgG1
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD54 / ICAM-1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD54 / ICAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. Low, but measureable presence of CD54 / ICAM-1 could be seen in cells in the seminiferous ducts in testis, germinal center cells in the lymph node, glandular cells in the fallopian tube and urothelial cells in the urinary bladder. We were unable to detect CD54 / ICAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD54 / ICAM-1 expression as measured by anti-CD54 / ICAM-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - + - - - - + - - - - - - - -
ICAM1 Variability ++ + ++ + + + ++ ++ ++ + ++ ++ + + ++ ++ + + + +

Selected References

Sommaggio R, Cohnen A, Watzl C, and Costa C. 2012. J. Immunol. 188: 2075-2083. (in vitro blocking - Pig)Avril M, Tripathi AK, Brazier AJ, Andisi C, Janes JH, Soma VL, Sullivan DJ, Bull PC, Stins MF, and Smith JD. 2012. Proc. Natl. Acad. Sci. 109: E1782-E1790. (in vitro blocking)Dryden NH, Sperone A, Martin-Almedina S, Hannah RL, Birdsey GM, Khan ST, Layhadi JA et al. 2012. J. Biol. Chem. 287: 12331-12342. (Western Blot)Di Lorenzo A, Manes TD, Davalos A, Wright PL, and Sessa WC. 2011. Blood. 117: 2284-2295. (in vitro activation/cross-linking)Kim S, and Nadel JA. 2009. Am. J. Physiol. Lung Cell. Mol. Physiol. 297: L174-L183. (in vitro blocking, Western Blot)Goto E, Kohrogi H, Hirata N, Tsumori K, Hirosako S, Hamamoto J, Fujii K, Kawano O, and Ando M. 2000. Am. J. Respir. Cell Mol. Biol. 22: 405-411. (Immunohistochemistry - frozen tissue)

Limitations and Warranty

enQuire Bio's Human Anti-CD54 / ICAM-1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD54 / ICAM-1 Antibody [15.2]