Formalin-fixed, paraffin-embedded human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).
Formalin-fixed, paraffin-embedded human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).

Anti-CD54 / ICAM-1 Antibody Clone W-CAM-1; same as Wehi-CAM-1 or 1H4

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3383-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3383-MSM1-P1
CD54 / ICAM-1 General Information
Alternate Names
ICAM-1, Intercellular Adhesion Molecule 1, CD54, Cluster of Differentiation 54, ICAM1
Molecular Weight
85-115kDa
Chromosomal Location
19p13.3-p13.2
Curated Database and Bioinformatic Data
Gene SymbolICAM1
Entrez Gene ID3383
Ensemble Gene IDENSG00000090339
RefSeq Protein Accession(s)NP_000192
RefSeq mRNA Accession(s)NM_000201
RefSeq Genomic Accession(s)NC_000019, NG_012083, NC_018930
UniProt ID(s)P05362
UniGene ID(s)P05362
HGNC ID(s)5344
Cosmic ID(s)ICAM1
KEGG Gene ID(s)hsa:3383
PharmGKB ID(s)PA29592
General Description of CD54 / ICAM-1.
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC), on a variety of other cell types including activated fibroblasts, EC, macrophages,, lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1), to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43, to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1, blocks homotypic binding of purified populations of activated T-, B-lymphocytes, also aggregation of mixed T-, B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function, Ig production).

Human Anti-CD54 / ICAM-1 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD54 / ICAM-1 Antibody Clone: W-CAM-1; same as Wehi-CAM-1 or 1H4
Clone W-CAM-1; same as Wehi-CAM-1 or 1H4 Host and Isotype: Mouse IgG2b kappa
Anti-Human CD54 / ICAM-1 Positive Control Sample: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Raji Burkitt lymphoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD54 / ICAM-1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD54 / ICAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. Low, but measureable presence of CD54 / ICAM-1 could be seen in cells in the seminiferous ducts in testis, germinal center cells in the lymph node, glandular cells in the fallopian tube and urothelial cells in the urinary bladder. We were unable to detect CD54 / ICAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD54 / ICAM-1 expression as measured by anti-CD54 / ICAM-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - + - - - - + - - - - - - - -
ICAM1 Variability ++ + ++ + + + ++ ++ ++ + ++ ++ + + ++ ++ + + + +

Limitations and Warranty

enQuire Bio's CD54 / ICAM-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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