Human Anti-CD54 / ICAM-1 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD54 / ICAM-1 Antibody Clone: W-CAM-1; same as Wehi-CAM-1 or 1H4
Clone W-CAM-1; same as Wehi-CAM-1 or 1H4 Host and Isotype: Mouse IgG2b kappa
Anti-Human CD54 / ICAM-1 Positive Control Sample: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Raji Burkitt lymphoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD54 / ICAM-1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD54 / ICAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, endothelial cells in the colon and pneumocytes in lung. Low, but measureable presence of CD54 / ICAM-1 could be seen in cells in the seminiferous ducts in testis, germinal center cells in the lymph node, glandular cells in the fallopian tube and urothelial cells in the urinary bladder. We were unable to detect CD54 / ICAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD54 / ICAM-1 expression as measured by anti-CD54 / ICAM-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | - | + | - | - | - | - | + | - | - | - | - | - | - | - | - |
ICAM1 Variability | ++ | + | ++ | + | + | + | ++ | ++ | ++ | + | ++ | ++ | + | + | ++ | ++ | + | + | + | + |
Limitations and Warranty
enQuire Bio's CD54 / ICAM-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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