Human and Rat Anti-Moesin Antibody Product Attributes
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Moesin Antibody Clone: SPM562
Clone SPM562 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat Moesin Positive Control Sample: HT-29, CH3LC, or HUVEC cells. Uterus, placenta, tonsil (both B, T lymphocytes), skeletal muscle, thyroid, or kidney.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Moesin protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Moesin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MSN antibody staining is expected to be primarily localized to the plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in Moesin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, germinal center cells in the lymph node and tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, germinal center cells in the lymph node and tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of Moesin could be seen inglandular cells in the breast, cells in the molecular layer in cerebellum, glial cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, cells in the endometrial stroma in endometrium, glandular cells in the endometrium and fallopian tube, follicle cells in the ovary, ovarian stroma cells in the ovary, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland, prostate and seminal vesicle, fibroblasts in skin, keratinocytes in skin, melanocytes in skin, smooth muscle cells in the smooth muscle, cells in the seminiferous ducts in testis, glandular cells in the thyroid gland and urothelial cells in the urinary bladder. We were unable to detect Moesin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Moesin expression as measured by anti-Moesin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | ++ | - | + | - | +++ | + | ++ | +++ | ++ | + | ++ | - | ++ | ++ | - | + | +++ | - |
| MSN Variability | + | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | + | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's Moesin Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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