Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-) Anti-NGF-Receptor / CD271 Antibody Product Attributes
Species: Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-NGF-Receptor / CD271 Antibody Clone: SPM299
Clone SPM299 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-) NGF-Receptor / CD271 Positive Control Sample: Neuronal axons, Schwann cells,, perineural cells of peripheral nerves, or tumors of nerve sheath differentiation e.g. Schwannoma, Neurofibroma. Soma, axons of sensory neurons,, ganglionic satellite cells. Melanomas.
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: NGFR from A875 melanoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
NGF-Receptor / CD271 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-NGFR antibody staining is expected to be primarily localized to the nucleoplasm and plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in NGF-Receptor / CD271 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in peripheral nerve/ganglion in colon. Low, but measureable presence of NGF-Receptor / CD271 could be seen inrespiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine and esophagus, glandular cells in the gallbladder, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin and squamous epithelial cells in the tonsil. We were unable to detect NGF-Receptor / CD271 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of NGF-Receptor / CD271 expression as measured by anti-NGF-Receptor / CD271 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
NGFR Variability | + | ++ | + | + | + | ++ | ++ | + | + | ++ | + | + | + | + | + | ++ | + | + | + | ++ |
Limitations and Warranty
enQuire Bio's NGF-Receptor / CD271 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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