Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1462).
Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1462).Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1462).

Anti-NSE gamma Antibody Clone ENO2/1462

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2026-MSM2
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 2026-MSM2-P0
NSE gamma General Information
Alternate Names
Gamma-enolase enolase 2, ENO2, neuron specific enolase, NSE,
Molecular Weight
50kDa
Chromosomal Location
12p13
Curated Database and Bioinformatic Data
Gene SymbolENO2
Entrez Gene ID2026
Ensemble Gene IDENSG00000111674
RefSeq Protein Accession(s)NP_001966
RefSeq mRNA Accession(s)NM_001975
RefSeq Genomic Accession(s)NC_000012, NC_018923
UniProt ID(s)P09104, Q6FHV6
UniGene ID(s)P09104, Q6FHV6
HGNC ID(s)3353
Cosmic ID(s)ENO2
KEGG Gene ID(s)hsa:2026
PharmGKB ID(s)PA27788
General Description of NSE gamma.
Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Human Anti-NSE gamma Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-NSE gamma Antibody Clone: ENO2/1462
Clone ENO2/1462 Host and Isotype: Mouse IgG2b
Anti-Human NSE gamma Positive Control Sample: HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide corresponding to aa416-433 of human NSE gamma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

NSE gamma Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ENO2 antibody staining is expected to be primarily localized to the cytosol, nucleus and plasma membrane. There is variability in either the signal strength or the localization of signal in cytosol and nucleus from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in NSE gamma antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the granular layer in cerebellum, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus and neuropil in cerebral cortex. More moderate antibody staining intensity was present in cells in the granular layer in cerebellum, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus and neuropil in cerebral cortex. Low, but measureable presence of NSE gamma could be seen inglial cells in the cerebral cortex and peripheral nerve in mesenchymal tissue. We were unable to detect NSE gamma in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of NSE gamma expression as measured by anti-NSE gamma antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - ++ - - - - - - - - - - - - - - - + - -
ENO2 Variability + + + + + ++ + + + + + + + + ++ + + +++ ++ +

Limitations and Warranty

enQuire Bio's NSE gamma Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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