Human SHH / Sonic hedgehog Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant SHH / Sonic hedgehog based upon sequence from: Human
Host: QP10181 protein expressed in E. coli.
Protein Construction: A DNA sequence encoding the Human SHH / Sonic hedgehog, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10181.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 ug/ml, corresponding to a specific activity of > 1.0 Ã 103 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Concentration of Human SHH / Sonic hedgehog Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered 20 mM PBS, pH 7.4, 150mM NaCl, lyophilized
Storage Conditions: Store at -20C to -80C.
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.