Recombinant Human TNF-alpha Protein

$ 98.00$ 718.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10354
Species: Human
Available Tags: Untagged
Host: E. coli
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Length (Amino Acids): 158

Product NumberDescriptionPrice
QP10354-ec-50ug Size: 50 ug, Tag: Untagged (Native Protein) $ 248.00
QP10354-ec-100ug Size: 100 ug, Tag: Untagged (Native Protein) $ 408.00
QP10354-ec-250ug Size: 250 ug, Tag: Untagged (Native Protein) $ 718.00
QP10354-ec-10ug Size: 10 ug, Tag: Untagged (Native Protein) $ 98.00
Shipping Information
7-10 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP10354-ec-50ug
Recombinant Human TNF-alpha Protein General Information
Alternate Names
TNF-alpha; TNF-a; Cachectin; TNFSF2; TNF-alpha; TNFA
Curated Database and Bioinformatic Data
Gene SymbolTNF
Entrez Gene ID7124
Ensemble Gene IDENSG00000204490
RefSeq Protein Accession(s)NP_000585.2
RefSeq mRNA Accession(s)NM_000594.3
UniProt ID(s)P01375
UniGene ID(s)Hs.241570
HGNC ID(s)HGNC:11892
COSMIC ID Link(s)TNF
KEGG Gene ID(s)hsa:7124
PharmGKB ID(s)PA435
General Description of Recombinant Human TNF-alpha Protein.
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). ; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Human TNF-alpha Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant TNF-alpha based upon sequence from: Human
Host: QP10354 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human TNF-alpha, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10354.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human TNF-alpha Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0. 2um filtered PBS, pH7.4, lyophilized
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human TNF-alpha Protein