Human Anti-IgM Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-IgM Antibody Clone: SPM557
Clone SPM557 Host and Isotype: Mouse IgG1 kappa
Anti-Human IgM Positive Control Sample: 293T, Raji or hPBL cells. Tonsil or Spleen.
Cellular Localization of Antibody
Cytoplasm, Cell Surface, Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant heavy chain of human IgM
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
IgM Previously Observed Antibody Staining Patterns
|IgM General Information|
|Ig mu chain C region, IGHM|
|Curated Database and Bioinformatic Data|
|Entrez Gene ID||3507|
|RefSeq Genomic Accession(s)||NC_000014, NG_187600, NC_018925, NG_001019|
|General Description of IgM.|
|Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas,, certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal, therefore malignant.|
Limitations and Warranty
enQuire Bio’s IgM Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.