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Flow Cytometry Protocol

Prepare your cell suspensions for Flow Cytometry Although most flow cytometry experiments involve labeling populations of cells that are relatively abundant, the number of cells required will vary depending upon the rarity of your cells. In general, researchers will stain between 1 x 105 and 1 x 106 cells per sample. However, with exceeding rare...

Anti-CD4 Antibody [RM4-5]

SKU: QAB9
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;APC-Cy7 ;PerCP ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

Anti-CD28 Antibody [37.51]

SKU: QAB99 Species: Mouse Tested Applications: Flow Cytometry, immunoprecipitation Available Conjugates: Unconjugated Isotype: Golden Syrian Hamster IgG Mass Spec Validated?: Not MS Validated

Anti-CD279 / PD-1 Antibody [J43.1]

SKU: QAB98
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE ;Unconjugated
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

Human lysed whole blood was stained with 5 uL (right panel) or 0.5 ug PerCP Mouse IgG1 isotype control (left panel).

Anti-CD45 Antibody [2D1]

SKU: QAB97
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE ;PerCP ;PerCP-Cy5.5 ;PE-Cy5 ;PE-Cy7
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Anti-IL-12 / IL-23 p40 Antibody [C17.8]

SKU: QAB96
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

Anti-CD274 / PD-L1 Antibody [10F.9G2]

SKU: QAB95
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE ;Unconjugated
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

Anti-CD86 Antibody [IT2.2]

SKU: QAB94
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Anti-CD86 Antibody [PO3.1]

SKU: QAB93
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

Anti-CD38 Antibody [HB7]

SKU: QAB92
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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