SDS-PAGE separation of QP8605 followed by commassie total protein stain results in a primary band consistent with reported data for TNF-alpha. These data demonstrate Greater than 90% as determined by SDS-PAGE.

Recombinant Bovine TNF-alpha Protein

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SKU: QP8605
Species: Bovine
Available Tags: GST
Host: E. coli
Purity: Greater than 90% as determined by SDS-PAGE.
Length (Amino Acids): 157

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On Demand Protein: 3-5 Week Lead Time. (Why?)
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP8605-ec-10ug
Recombinant Bovine TNF-alpha Protein General Information
Curated Database and Bioinformatic Data
Gene SymbolTNF
Entrez Gene ID280943
Ensemble Gene IDENSBTAG00000025471
RefSeq Protein Accession(s)NP_776391.2
RefSeq mRNA Accession(s)NM_173966.3
UniProt ID(s)Q06599
UniGene ID(s)Bt.12756
COSMIC ID Link(s)TNF
KEGG Gene ID(s)bta:280943
General Description of Recombinant Bovine TNF-alpha Protein.
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Bovine TNF-alpha Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant TNF-alpha based upon sequence from: Bovine
Host: QP8605 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Bos taurus (Bovine) TNF-alpha, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8605.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Leu78 - Leu234
Amino Acid Sequence: LRSSSQASSN KPVAHVVADI NSPGQLRWWD SYANALMANG VKLEDNQLVV PADGLYLIYS QVLFRGQGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETPEWAE AKPWYEPIYQ GGVFQLEKGD RLSAEINLPD YLDYAESGQV YFGIIAL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Bovine TNF-alpha Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.

Limitations and Performance Guarantee

This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Bovine TNF-alpha Protein