PDF DatasheetAntibody DISCONTINUED
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at RoomTemperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-CD3 Antibody Clone: EP41
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Tonsil, Lymphoma
Cellular Localization of Antibody EP41 Staining: Cell Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues in cytoplasmic domain of human CD3 epsilon protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CD3 Information for Pathologists
| CD3 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 23.1 kDa | |
| Chromosomal Location | |
| q23.3 [chr: 11] [chr_start: 118304545] [chr_end: 118316175] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD3E |
| Entrez Gene ID | 916 |
| RefSeq Protein Accession(s) | NP_000724 |
| RefSeq mRNA Accession(s) | NM_000733; |
| RefSeq Genomic Accession(s) | NG_007383; NC_000011 |
| UniProt ID(s) | P07766 |
| PharmGKB ID(s) | PA26216 |
| KEGG Gene ID(s) | hsa:916 |
| Associated Diseases (KEGG IDs) | Immunodeficiency 18 (IMD18) [MIM:615615]: An autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood of recurrent infections. The severity is variable, encompassing both a mild immunodeficiency and severe combined immunodeficiency (SCID), resulting in early death without bone marrow transplantation in some patients. Immunologic work-up of the IMD18 SCID patients shows a T cell-negative, B cell-positive, natural killer (NK) cell-positive phenotype, whereas T-cell development is not impaired in the mild form of IMD18. {ECO:0000269|PubMed:15546002, ECO:0000269|PubMed:8490660}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of CD3 . | |
| CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The CD3 is involved in T cell development and survival. It is expressed on T cells in Thymus, peripheral lymphoid tissue, blood and bone marrow. CD3 is a commonly used marker for identification of T cell and T cell derived malignancies. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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