PDF DatasheetHuman, Monkey, Cow, Pig, Goat, Rat, Mouse, Guinea pig, and Frog Anti-Cytokeratin Antibody Product Attributes
Species: Human, Monkey, Cow, Pig, Goat, Rat, Mouse, Guinea pig, and Frog
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: KRT/457
Clone KRT/457 Host and Isotype: Mouse IgG1
Anti-Human, Monkey, Cow, Pig, Goat, Rat, Mouse, Guinea pig, and Frog Cytokeratin Positive Control Sample: A431 cells, Skin, Colon carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Keratin-enriched preparation from cultured human epithelial cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-KRT4 antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.
| Cytokeratin General Information | |
|---|---|
| Molecular Weight | |
| Multiple | |
| Chromosomal Location | |
| Multiple | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Multiple |
| Entrez Gene ID | 3851 |
| Ensemble Gene ID | ENSG00000170477 |
| RefSeq Protein Accession(s) | NP_002263 |
| RefSeq mRNA Accession(s) | NM_002272, |
| RefSeq Genomic Accession(s) | NG_007380, NC_000012, NC_018923 |
| UniProt ID(s) | B4DRS2 |
| UniGene ID(s) | B4DRS2 |
| HGNC ID(s) | 6441 |
| Cosmic ID(s) | KRT4 |
| KEGG Gene ID(s) | hsa:3851 |
| General Description of Cytokeratin. | |
| Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW), basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13), 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research, tumor diagnosis. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin, Multi Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.