PDF DatasheetHuman Anti-HLA-DRB Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-HLA-DRB Antibody Clone: SPM289
Clone SPM289 Host and Isotype: Mouse IgG2b kappa
Anti-Human HLA-DRB Positive Control Sample: Ramos, Daudi or HuT78 cells. Tonsil or lymph node
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Activated human peripheral blood mononuclear cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
HLA-DRB Previously Observed Antibody Staining Patterns
| HLA-DRB General Information | |
|---|---|
| Alternate Names | |
| Human Leukocyte Antigen, Antigen D Related, MHC Class II, HLADR, HLA-DR | |
| Molecular Weight | |
| ~28kDa (beta chain) | |
| Chromosomal Location | |
| 6p21.3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HLA-DR |
| Entrez Gene ID | 3123 |
| Ensemble Gene ID | ENSG00000206240, ENSG00000236884, ENSG00000206306, ENSG00000228080, ENSG00000229074, ENSG00000196126 |
| RefSeq Protein Accession(s) | XP_011546040, NP_001230894, NP_002115 |
| RefSeq mRNA Accession(s) | NM_001243965, XM_011547738, NM_002124 |
| RefSeq Genomic Accession(s) | NG_029921, NG_002392, NG_167248, NC_018917, NG_002433, NC_000006, NG_002432, NG_113891, NG_167245, NG_167249, NG_167246 |
| UniProt ID(s) | Q29974, D7RIG5, P01912, X5DNQ0, D7RIH8, Q5Y7D1, P13761, P01911 |
| UniGene ID(s) | Q29974, D7RIG5, P01912, X5DNQ0, D7RIH8, Q5Y7D1, P13761, P01911 |
| HGNC ID(s) | 4948 |
| Cosmic ID(s) | HLA-DRB1 |
| KEGG Gene ID(s) | hsa:3123 |
| PharmGKB ID(s) | PA35072 |
| General Description of HLA-DRB. | |
| This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP, HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody,, these antibodies do not cross-block binding to each other’s respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain, a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells, other non-professional APCs. In conjunction with the CD3/TCR complex, CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells, their corresponding neoplastic counterparts. | |
Limitations and Warranty
enQuire Bio’s HLA-DRB Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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