PDF DatasheetHuman, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish Anti-TNF-alpha Antibody Product Attributes
Species: Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-paraffin) (2-4ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-TNF-alpha Antibody Clone: P/T2
Clone P/T2 Host and Isotype: Mouse IgM kappa
Anti-Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish TNF-alpha Positive Control Sample: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Cellular Localization of Antibody
Cytoplasmic, extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF-?, conjugated to thyroglobulin
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
TNF-alpha Previously Observed Antibody Staining Patterns
| TNF-alpha General Information | |
|---|---|
| Alternate Names | |
| Tumor necrosis factor, TNF, TNFalpha, TNFa, TNF alpha, TNF? | |
| Molecular Weight | |
| 17kDa | |
| Chromosomal Location | |
| 6p21.33 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TNF |
| Entrez Gene ID | 7124 |
| Ensemble Gene ID | ENSG00000232810, ENSG00000230108, ENSG00000223952, ENSG00000206439, ENSG00000228849, ENSG00000228978, ENSG00000228321, ENSG00000204490 |
| RefSeq Protein Accession(s) | NP_000585 |
| RefSeq mRNA Accession(s) | NM_000594 |
| RefSeq Genomic Accession(s) | NG_167244, NG_167248, NC_018917, NG_167246, NG_167247, NG_007462, NG_113891, NG_167245, NG_167249, NC_000006 |
| UniProt ID(s) | Q5STB3, P01375 |
| UniGene ID(s) | Q5STB3, P01375 |
| HGNC ID(s) | 11892 |
| Cosmic ID(s) | TNF |
| KEGG Gene ID(s) | hsa:7124 |
| PharmGKB ID(s) | PA435 |
| General Description of TNF-alpha. | |
| Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages,, causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock, tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors, cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts, other cell lines, activating polymorphonuclear neutrophils, osteoclasts,, induction of interleukin 1, prostaglandin E2, collagenase production. | |
Limitations and Warranty
enQuire Bio’s TNF-alpha Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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