PDF DatasheetHuman TNF-alpha Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TNF-alpha based upon sequence from: Human
Host: QP10354 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human TNF-alpha, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10354.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human TNF-alpha Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0. 2um filtered PBS, pH7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human TNF-alpha Protein General Information | |
|---|---|
| Alternate Names | |
| TNF-alpha; TNF-a; Cachectin; TNFSF2; TNF-alpha; TNFA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TNF |
| Entrez Gene ID | 7124 |
| Ensemble Gene ID | ENSG00000204490 |
| RefSeq Protein Accession(s) | NP_000585.2 |
| RefSeq mRNA Accession(s) | NM_000594.3 |
| UniProt ID(s) | P01375 |
| UniGene ID(s) | Hs.241570 |
| HGNC ID(s) | HGNC:11892 |
| COSMIC ID Link(s) | TNF |
| KEGG Gene ID(s) | hsa:7124 |
| PharmGKB ID(s) | PA435 |
| General Description of Recombinant Human TNF-alpha Protein. | |
| Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key ‘Ser-418’ residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). ; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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