PDF DatasheetHuman bFGF / FGF2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant bFGF / FGF2 based upon sequence from: Human
Host: QP5270 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5270.
Bioactivity Data: BioActivity: Shows bioactivity at a concentration of 5 ng/mL in the 3T3 cell proliferation assay. The bioactivity acceptance criteria is set at 2 x 10^5 units/mg.
Monomer or Dimer: Monomer
Amino Acid Sequence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human bFGF / FGF2 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Human bFGF / FGF2 Protein (Animal Free) General Information | |
|---|---|
| Alternate Names | |
| bFGF, FGF2, FGF-2, HBGF-2, BFGF, FGFB | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FGF2 |
| Entrez Gene ID | 2247 |
| Ensemble Gene ID | ENSG00000138685 |
| RefSeq Protein Accession(s) | NP_001997.5 |
| RefSeq mRNA Accession(s) | NM_002006.4 |
| UniProt ID(s) | P09038 |
| UniGene ID(s) | Hs.284244 |
| HGNC ID(s) | HGNC:3676 |
| COSMIC ID Link(s) | FGF2 |
| KEGG Gene ID(s) | hsa:2247 |
| PharmGKB ID(s) | PA28115 |
| General Description of Recombinant Human bFGF / FGF2 Protein (Animal Free). | |
| Basic fibroblast growth factor (bFGF), also known as FGF2, is expressed by endothelial cells and is a mediator of angiogenesis. bFGF also has cardioprotective functions during heart injury. The application of bFGF is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant bFGF-154aa is the full length bFGF protein encoded by the human FGF2 gene. There are no detectable differences in biological activity between bFGF-154aa and the truncated bFGF-147aa proteins. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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