PDF DatasheetHuman LIF Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LIF based upon sequence from: Human
Host: QP5361 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5361.
Bioactivity Data: BioActivity: Shows bioactivity at a concentration of 200 pg/mL in the TF-1 cell proliferation assay. The bioactivity acceptance criteria is set at 5 x 10^6 units/mg.
Monomer or Dimer: Monomer
Amino Acid Sequence: MSPLPITPVN ATCAIRHPCH NNLMNQIRSQ LAQLNGSANA LFILYYTAQG EPFPNNLDKL CGPNVTDFPP FHANGTEKAK LVELYRIVVY LGTSLGNITR DQKILNPSAL SLHSKLNATA DILRGLLSNV LCRLCSKYHV GHVDVTYGPD TSGKDVFQKK KLGCQLLGKY KQIIAVLAQA F
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human LIF Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: Sterile filtered (0.22 um) solution containing 0.1% TFA
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Human LIF Protein General Information | |
|---|---|
| Alternate Names | |
| LIF, MLPLI, CDF, DIA, HILDA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LIF |
| Entrez Gene ID | 3976 |
| Ensemble Gene ID | ENSG00000128342 |
| RefSeq Protein Accession(s) | NP_001244064.1 |
| RefSeq mRNA Accession(s) | NM_001257135.1, NM_002309.4 |
| UniProt ID(s) | P15018 |
| UniGene ID(s) | Hs.2250 |
| HGNC ID(s) | HGNC:6596 |
| COSMIC ID Link(s) | LIF |
| KEGG Gene ID(s) | hsa:3976 |
| PharmGKB ID(s) | PA30370 |
| General Description of Recombinant Human LIF Protein. | |
| Leukemia inhibitory factor (LIF) is a member of the interleukin 6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. Human LIF may also be an important factor during human embryonic stem cell (hESC) self-renewal, pluripotency, and embryonic implantation. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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