PDF DatasheetHuman LIF Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LIF based upon sequence from: Human
Host: QP10360 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human LIF, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10360.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human LIF Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human LIF Protein General Information | |
|---|---|
| Alternate Names | |
| LIF; MLPLI; CDF; DIA; HILDA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LIF |
| Entrez Gene ID | 3976 |
| Ensemble Gene ID | ENSG00000128342 |
| RefSeq Protein Accession(s) | NP_001244064.1 |
| RefSeq mRNA Accession(s) | NM_001257135.1, NM_002309.4 |
| UniProt ID(s) | P15018 |
| UniGene ID(s) | Hs.2250 |
| HGNC ID(s) | HGNC:6596 |
| COSMIC ID Link(s) | LIF |
| KEGG Gene ID(s) | hsa:3976 |
| PharmGKB ID(s) | PA30370 |
| General Description of Recombinant Human LIF Protein. | |
| LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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