PDF DatasheetMouse LIF Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LIF based upon sequence from: Mouse
Host: QP4235 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP4235.
Bioactivity Data: BioActivity: Shows bioactivity at a concentration of 1 ng/mL in the Inhibit M1 cell proliferation assay. The bioactivity acceptance criteria is set at 1 x 10^6 units/mg.
Monomer or Dimer: Monomer
Amino Acid Sequence: MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Mouse LIF Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: Sterile filtered (0.22 um) solution containing 0.1% TFA
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Mouse LIF Protein General Information | |
|---|---|
| Alternate Names | |
| LIF, MLPLI, CDF, DIA, HILDA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LIF |
| Entrez Gene ID | 16878 |
| Ensemble Gene ID | ENSMUSG00000034394 |
| RefSeq Protein Accession(s) | NP_001034626.1 |
| RefSeq mRNA Accession(s) | NM_001039537.2, NM_008501.2, XM_006514542.3, XM_006514543.3 |
| UniProt ID(s) | P09056 |
| UniGene ID(s) | Mm.4964 |
| COSMIC ID Link(s) | LIF |
| KEGG Gene ID(s) | mmu:16878 |
| General Description of Recombinant Mouse LIF Protein. | |
| Mouse leukemia inhibitory factor (LIF) is a member of the interleukin 6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. The application of mouse LIF to long-term culture systems promotes mouse embryonic stem (ES) cell self-renewal and pluripotency, similar to the functional activity of bFGF (FGF2) in human ES cell culture systems. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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