PDF DatasheetHuman PDGF-B Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PDGF-B based upon sequence from: Human
Host: QP5307 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5307.
Bioactivity Data: BioActivity: Shows bioactivity at a concentration of 20 ng/mL in the BALB/c 3T3 cell proliferation assay. The bioactivity acceptance criteria is set at 5.0 x 10^4 units/mg.
Monomer or Dimer: Dimer
Amino Acid Sequence: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human PDGF-B Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, pH 7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Human PDGF-B Protein General Information | |
|---|---|
| Alternate Names | |
| PDGF-B, IBGC5, PDGF2, SSV, PDGF-2, SIS, c-sis | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDGFB |
| Entrez Gene ID | 5155 |
| Ensemble Gene ID | ENSG00000100311 |
| RefSeq Protein Accession(s) | NP_002599.1 |
| RefSeq mRNA Accession(s) | NM_002608.3, NM_033016.3 |
| UniProt ID(s) | P01127 |
| UniGene ID(s) | Hs.1976 |
| HGNC ID(s) | HGNC:8800 |
| COSMIC ID Link(s) | PDGFB |
| KEGG Gene ID(s) | hsa:5155 |
| PharmGKB ID(s) | PA33145 |
| General Description of Recombinant Human PDGF-B Protein. | |
| Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-a, TGF-b and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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