PDF DatasheetMouse EGF / Epidermal Growth Factor Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant EGF / Epidermal Growth Factor based upon sequence from Mouse
Host: QP5482 protein expressed in E. coli.
Tag: Biotin
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5482.
Bioactivity Data: 3T3 cell proliferation significantly induced by less than 250 pg/ml of Mouse EGF / Epidermal Growth Factor (which is equivalent to activity of at least 4.0 x 10^6 units/mg or an ED50 typically around 10-100 pg/ml).
Monomer or Dimer: Monomer
Amino Acid Sequence: MKKIDDDKNS YPGCPSSYD G YCLNGGVC MH IESLDSY TCN CVIGYS GDRC QTRDL EWWEL R
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: A Certificate of Analysis (CofA) will be sent along with the protein and will contain the proper reconstitution instructions.
Concentration of Mouse EGF / Epidermal Growth Factor Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 0.5 mg/mL in sterile PBS
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Biotinylated Recombinant Mouse EGF Protein (Biotin) General Information | |
|---|---|
| Alternate Names | |
| EGF, Epidermal Growth Factor, URG, HOMG4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EGF |
| Entrez Gene ID | 13645 |
| Ensemble Gene ID | ENSMUSG00000028017 |
| RefSeq Protein Accession(s) | NP_034243.2 |
| RefSeq mRNA Accession(s) | NM_010113.4 |
| UniProt ID(s) | P01132 |
| UniGene ID(s) | Mm.252481 |
| COSMIC ID Link(s) | EGF |
| KEGG Gene ID(s) | mmu:13645 |
| General Description of Biotinylated Recombinant Mouse EGF Protein (Biotin). | |
| Epidermal growth factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR). The recombinant EGF-Biotin conjugate has an N-terminal leader sequence containing a biotin molecule. There is half of a biotin molecule per EGF protein. The biotin conjugate does not alter the biological activity of the EGF protein. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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