PDF DatasheetHuman EGF / Epidermal Growth Factor Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant EGF / Epidermal Growth Factor based upon sequence from: Human
Host: QP10394 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human EGF / Epidermal Growth Factor, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10394.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human EGF / Epidermal Growth Factor Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 m filtered 20 mM PBS, pH 7.2, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human EGF / Epidermal Growth Factor Protein General Information | |
|---|---|
| Alternate Names | |
| EGF; Epidermal Growth Factor; URG; HOMG4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EGF |
| Entrez Gene ID | 1950 |
| Ensemble Gene ID | ENSG00000138798 |
| RefSeq Protein Accession(s) | NP_001171601.1 |
| RefSeq mRNA Accession(s) | NM_001178130.2, NM_001178131.2, NM_001963.5 |
| UniProt ID(s) | P01133 |
| UniGene ID(s) | Hs.419815 |
| HGNC ID(s) | HGNC:3229 |
| COSMIC ID Link(s) | EGF |
| KEGG Gene ID(s) | hsa:1950 |
| PharmGKB ID(s) | PA27664 |
| General Description of Recombinant Human EGF / Epidermal Growth Factor Protein. | |
| EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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