PDF DatasheetHuman CXCL12 / SDF-1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CXCL12 / SDF-1 based upon sequence from: Human
Host: QP10217 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human CXCL12 / SDF-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10217.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human CXCL12 / SDF-1 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human CXCL12 / SDF-1 Protein General Information | |
|---|---|
| Alternate Names | |
| CXCL12; SDF-1; SCYB12; IRH; PBSF; SDF1; TPAR1; TLSF | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CXCL12 |
| Entrez Gene ID | 6387 |
| Ensemble Gene ID | ENSG00000107562 |
| RefSeq Protein Accession(s) | NP_000600.1 |
| RefSeq mRNA Accession(s) | NM_000609.6, NM_001033886.2, NM_001178134.1, NM_001277990.1, NM_199168.3 |
| UniProt ID(s) | P48061 |
| UniGene ID(s) | Hs.522891 |
| HGNC ID(s) | HGNC:10672 |
| COSMIC ID Link(s) | CXCL12 |
| KEGG Gene ID(s) | hsa:6387 |
| PharmGKB ID(s) | PA35602 |
| General Description of Recombinant Human CXCL12 / SDF-1 Protein. | |
| Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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