PDF DatasheetMouse CXCL12 / SDF-1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CXCL12 / SDF-1 based upon sequence from: Mouse
Host: QP10259 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Mouse CXCL12 / SDF-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10259.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRL KM
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Mouse CXCL12 / SDF-1 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse CXCL12 / SDF-1 Protein General Information | |
|---|---|
| Alternate Names | |
| CXCL12; SDF-1; SCYB12; IRH; PBSF; SDF1; TPAR1; TLSF | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CXCL12 |
| Entrez Gene ID | 20315 |
| Ensemble Gene ID | ENSMUSG00000061353 |
| RefSeq Protein Accession(s) | NP_038683.1 |
| RefSeq mRNA Accession(s) | NM_013655.4, NM_021704.3 |
| UniProt ID(s) | P40224 |
| UniGene ID(s) | Mm.303231 |
| COSMIC ID Link(s) | CXCL12 |
| KEGG Gene ID(s) | mmu:20315 |
| General Description of Recombinant Mouse CXCL12 / SDF-1 Protein. | |
| Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells (By similarity). Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. ; Stimulates the proliferation of bone marrow-derived b progenitor cells in the presence of IL-7 as well as growth of the stromal cell-dependent B-cell clone DW34 cells. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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