PDF DatasheetHuman SCF / C-kit ligand Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant SCF / C-kit ligand based upon sequence from: Human
Host: QP10344 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human SCF / C-kit ligand, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10344.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA
Purity: Greater than 97% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human SCF / C-kit ligand Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human SCF / C-kit ligand Protein General Information | |
|---|---|
| Alternate Names | |
| SCF; C-kit ligand; FPH2; DCUA; Kitl; SHEP7; SCF; DFNA69; SF; FPHH; KL-1; MGF | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Kitlg |
| Entrez Gene ID | 4254 |
| Ensemble Gene ID | ENSG00000049130 |
| RefSeq Protein Accession(s) | NP_000890.1 |
| RefSeq mRNA Accession(s) | NM_000899.4, NM_003994.5 |
| UniProt ID(s) | P21583 |
| UniGene ID(s) | Hs.1048 |
| HGNC ID(s) | HGNC:6343 |
| COSMIC ID Link(s) | KITLG |
| KEGG Gene ID(s) | hsa:4254 |
| PharmGKB ID(s) | PA30129 |
| General Description of Recombinant Human SCF / C-kit ligand Protein. | |
| Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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