PDF DatasheetPufferfish Leptin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Leptin based upon sequence from Pufferfish
Host: QP10749 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Leptin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Pufferfish Leptin in sterile 0.4% NaHCO3 pH 9 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The affinity of human leptin receptors is considerably lower campared to mammalian leptins.
Monomer or Dimer: Dimer
Amino Acid Sequence: MDHILALVLA LLPLSLCVAL PGALDAMDVE KMKSKVTWKA QGLVARIDKH FPDRGLRFDT DKVEGSTSVV ASLESYNNLI SDRFGGVSQI KTEISSLAGY LNHWREGNCQ EQQPKVWPRR NIFNHTVSLE ALMRVREFLK LLQKNVDLLE RC
Purity: Greater than 99.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Pufferfish Leptin Protein: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Pufferfish Leptin was lyophilized from a concentrated (0.85 mg/ml) solution with 0.003mM NaHCO3.
Storage Conditions: Lyophilized Pufferfish Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Pufferfish Leptin General Information | |
|---|---|
| Alternate Names | |
| OB Protein, Obesity Protein, OBS, Obesity factor. | |
| Molecular Weight | |
| 16 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q588G0 |
| General Description of Recombinant Pufferfish Leptin. | |
| Leptin Pufferfish (Takifugu rubripes) Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 16 kDa. Bioactive Leptin Pufferfish (Takifugu rubripes) Recombinant was prepared according to the sequence published by Kurokawa et al. (2005)Peptides 26.745-750 in two forms: monomer and covalent dimer. MS analysis revealed molecular masses of 15,291 and 30,585 Da, close to the theoretical values of 15,270 and 30,540 Da. CD spectra revealed high similarity to mammalian leptins. Other details of its preparation will be soon published by Yacobovitz et al (in press), General and Comparative Endocrinology. The Pufferfish Leptin is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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