PDF DatasheetMouse Leptin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Leptin based upon sequence from Mouse
Host: QP10756 protein expressed in E. Coli.
Available Tags: 20kD PEG, Untagged
Protein Construction: A cDNA sequence encoding the sequence of Leptin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Leptin in sterile 0.4% NaHCO3 adjusted to pH 8.5 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Pegylated mouse Leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Pegylated mouse Leptin in vitro activity is only slightly lower than the non-pegylated antagonist but in vivo it has profound weight reducing effect (as compared to the non-pegylated leptin), resulting mainly from reduced food intake.
Amino Acid Sequence: MCWRPLCRFL WLWSYLSYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
Purity: Greater than 99.0% as determined by:(a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The mouse Leptin was lyophilized from a concentrated (0.65 mg/ml) solution containing 0.003mM NaHCO3.
Storage Conditions: Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse Leptin General Information | |
|---|---|
| Alternate Names | |
| OB Protein, Obesity Protein, OBS, Obesity factor. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P41160 |
| General Description of Recombinant Mouse Leptin. | |
| Mouse Leptin Mono-PEGylated produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and an additional Ala at N-terminus. PEGylated Mouse Leptin contains PEG 20 kDa at its N-terminus and having a molecular mass of 35.6 kDa as determined by mass spectrometry. Since its enlarged hydrodynamic volume PEGylated Leptin runs on SDS-PAGE as A 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. PEGylated Mouse Leptin half-life in circulation after SC injection was over 20 hours. Mouse Leptin was purified by using an optimized multi-step FPLC method for maximum separation from contaminants. according to Salomon et al (2006) Protein Expression and Purification 47.128136 and then PEGylated. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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