PDF DatasheetMouse Bax Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Bax based upon sequence from Mouse
Host: QP11131 protein expressed in E. Coli.
Available Tags: Untagged, GST
Protein Construction: A cDNA sequence encoding the sequence of Bax was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11131.
Bioactivity Data: Untested
Amino Acid Sequence: MDGSGEQLGS GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV WIQDQGGWEG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein contains 10mM Tris-HCL pH 8.0, 1mM EDTA and 250mM NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Mouse Bax General Information | |
|---|---|
| Alternate Names | |
| Apoptosis regulator BAX membrane isoform alpha, Bax, Bcl2-associated X protein. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q07813 |
| General Description of Recombinant Mouse Bax. | |
| Mouse Bax amino acid 38-171 produced in E. Coli is a single, non-glycosylated polypeptide chain. The Mouse Bax is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
1.Bax Regulates Production of Superoxide in Both Apoptotic and Nonapoptotic Neurons: Role of Caspases.Publication:The Journal of Neuroscience, 1 December 2010, 30(48): 16114-16127; doi: 10.1523/?JNEUROSCI.2862-10.2010 The Journal of Neuroscience, 1 December 2010, 30(48): 16114-16127; doi: 10.1523/?JNEUROSCI.2862-10.2010 Link:http://www.jneurosci.org/content/30/48/16114.full.pdf2.Interaction of IGF-binding Protein-3 and BAX in Mitochondria Promotes Male Germ Cell ApoptosisPublication:First Published on November 3, 2009, doi: 10.1074/jbc.M109.046847 January 15, 2010 The Journal of Biological Chemistry, 285, 1726-1732. Link:http://www.jbc.org/content/285/3/1726.full
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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