PDF DatasheetChicken GAPDH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GAPDH based upon sequence from Chicken
Host: QP6069 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Gallus gallus (Chicken) GAPDH, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6069.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Val2 – Glu333
Amino Acid Sequence: VKVGVNGFGR IGRLVTRAAV LSGKVQVVAI NDPFIDLNYM VYMFKYDSTH GHFKGTVKAE NGKLVINGHA ITIFQERDPS NIKWADAGAE YVVESTGVFT TMEKAGAHLK GGAKRVIISA PSADAPMFVM GVNHEKYDKS LKIVSNASCT TNCLAPLAKV IHDNFGIVEG LMTTVHAITA TQKTVDGPSG KLWRDGRGAA QNIIPASTGA AKAVGKVIPE LNGKLTGMAF RVPTPNVSVV DLTCRLEKPA KYDDIKRVVK AAADGPLKGI LGYTEDQVVS CDFNGDSHSS TFDAGAGIAL NDHFVKLVSW YDNEFGYSNR VVDLMVHMAS KE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Chicken GAPDH Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Chicken GAPDH Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GAPDH |
| Entrez Gene ID | 374193 |
| Ensemble Gene ID | ENSGALG00000014442 |
| RefSeq Protein Accession(s) | NP_989636.1 |
| RefSeq mRNA Accession(s) | NM_204305.1 |
| UniProt ID(s) | P00356 |
| UniGene ID(s) | Gga.6383 |
| COSMIC ID Link(s) | GAPDH |
| KEGG Gene ID(s) | gga:374193 |
| General Description of Recombinant Chicken GAPDH Protein. | |
| Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assbly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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