PDF DatasheetHuman GAPDH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GAPDH based upon sequence from: Human
Host: QP8304 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) GAPDH, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8304.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Lys3 – Glu335
Amino Acid Sequence: KVKVGVNGFG RIGRLVTRAA FNSGKVDIVA INDPFIDLNY MVYMFQYDST HGKFHGTVKA ENGKLVINGN PITIFQERDP SKIKWGDAGA EYVVESTGVF TTMEKAGAHL QGGAKRVIIS APSADAPMFV MGVNHEKYDN SLKIISNASC TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA LQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTANVSV VDLTCRLEKP AKYDDIKKVV KQASEGPLKG ILGYTEHQVV SSDFNSDTHS STFDAGAGIA LNDHFVKLIS WYDNEFGYSN RVVDLMAHMA SKE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human GAPDH Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human GAPDH Protein (3-335aa) General Information | |
|---|---|
| Alternate Names | |
| GAPDH; G3PD; HEL-S-162eP; GAPD | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GAPDH |
| Entrez Gene ID | 2597 |
| Ensemble Gene ID | ENSG00000111640 |
| RefSeq Protein Accession(s) | NP_001243728.1 |
| RefSeq mRNA Accession(s) | NM_001256799.2, NM_001289745.1, NM_001289746.1, NM_002046.5 |
| UniProt ID(s) | P04406 |
| UniGene ID(s) | Hs.544577 |
| HGNC ID(s) | HGNC:4141 |
| COSMIC ID Link(s) | GAPDH |
| KEGG Gene ID(s) | hsa:2597 |
| PharmGKB ID(s) | PA28554 |
| General Description of Recombinant Human GAPDH Protein (3-335aa). | |
| Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assbly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and mbrane associations through its ability to stimulate the binding of CHP1 to microtubules . Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assbles into the GAIT complex which binds to st loop-containing GAIT elents in the 3′-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.3 Publications | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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