PDF DatasheetHuman GAPDH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GAPDH based upon sequence from Human
Host: QP6070 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) GAPDH, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP6070.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Gly2 – Glu335
Amino Acid Sequence: GKVKVGVNGF GRIGRLVTRA AFNSGKVDIV AINDPFIDLN YMVYMFQYDS THGKFHGTVK AENGKLVING NPITIFQERD PSKIKWGDAG AEYVVESTGV FTTMEKAGAH LQGGAKRVII SAPSADAPMF VMGVNHEKYD NSLKIISNAS CTTNCLAPLA KVIHDNFGIV EGLMTTVHAI TATQKTVDGP SGKLWRDGRG ALQNIIPAST GAAKAVGKVI PELNGKLTGM AFRVPTANVS VVDLTCRLEK PAKYDDIKKV VKQASEGPLK GILGYTEHQV VSSDFNSDTH SSTFDAGAGI ALNDHFVKLI SWYDNEFGYS NRVVDLMAHM ASKE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human GAPDH Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human GAPDH Protein (2-335aa) General Information | |
|---|---|
| Alternate Names | |
| GAPDH, G3PD, HEL-S-162eP, GAPD | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GAPDH |
| Entrez Gene ID | 2597 |
| Ensemble Gene ID | ENSG00000111640 |
| RefSeq Protein Accession(s) | NP_001243728.1 |
| RefSeq mRNA Accession(s) | NM_001256799.2, NM_001289745.1, NM_001289746.1, NM_002046.5 |
| UniProt ID(s) | P04406 |
| UniGene ID(s) | Hs.544577 |
| HGNC ID(s) | HGNC:4141 |
| COSMIC ID Link(s) | GAPDH |
| KEGG Gene ID(s) | hsa:2597 |
| PharmGKB ID(s) | PA28554 |
| General Description of Recombinant Human GAPDH Protein (2-335aa). | |
| Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3′-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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