Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | No Pretreatment Required |
| Incubation Parameters | 30 min at RoomTemperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Polyclonal
Anti-alpha-1-Antichymotrypsin Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: alpha-1-antichymotrypsin isolated from human serum.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
alpha-1-Antichymotrypsin Information for Pathologists
Summary:
Also called ?1-ACT. Acute phase protein / plasma protease inhibitor, mainly produced by liver in response to cytokines such as oncostatin M. May limit tissue damage produced by excessive inflammation associated proteolysis. Also localizes to nuclei of hepatic cells to control chromatin condensation and proliferation (Gastroenterology 2013;144:818). Homologous to alpha-1-antitrypsin.Notable Clinical Features:
Heart: has role in pathophysiology of heart failure (Circ Heart Fail 2013;6:853). Kidney: part of plasma molecular profile associated with acute cellular renal allograft rejection (Transplantation 2011;92:388). Skin: has role in skin repair (J Biol Chem 2011;286:28889). Umbilical cord: may help differentiate between stillborns and liveborns (Pathol Res Pract 2009;205:534). Uses by pathologistsCommon Uses By Pathologists:
Marker for acinic / acinar cell carcinoma in breast (Case Rep Oncol Med 2013;2013:372947), pancreas, salivary gland.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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