Human Anti-CD79a Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Anti-CD79a Antibody Clone: SPM549
Clone SPM549 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD79a Positive Control Sample: Daudi or Ramos cells. Germinal center B-cells in a lymph node or tonsil.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD79a Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD79a antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen, germinal center cells in the lymph node, lymphoid tissue in appendix and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen, germinal center cells in the lymph node, lymphoid tissue in appendix and non-germinal center cells in the tonsil. Low, but measureable presence of CD79a could be seen inmacrophages in lung. We were unable to detect CD79a in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's CD79a Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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