Formalin-fixed, paraffin-embedded human Skin Stained with Cytokeratin 15 Mouse Monoclonal Antibody (SPM19).
Formalin-fixed, paraffin-embedded human Skin Stained with Cytokeratin 15 Mouse Monoclonal Antibody (SPM19).

Anti-Cytokeratin 15 Antibody Clone SPM190

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3866-MSM1X
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3866-MSM1X-P0
Cytokeratin 15 General Information
Alternate Names
Keratin-15, Cytokeratin-15, Keratin 15, KRT15
Molecular Weight
52kDa
Chromosomal Location
17q21.2
Curated Database and Bioinformatic Data
Gene SymbolKRT15
Entrez Gene ID3866
Ensemble Gene IDENSG00000171346
RefSeq Protein Accession(s)XP_011523086, NP_002266, XP_016880103
RefSeq mRNA Accession(s)XM_011524784, XM_017024614, NM_002275
RefSeq Genomic Accession(s)NG_012284, NC_000017, NC_018928
UniProt ID(s)P19012, B3KVF5
UniGene ID(s)P19012, B3KVF5
HGNC ID(s)6421
Cosmic ID(s)KRT15
KEGG Gene ID(s)hsa:3866
PharmGKB ID(s)PA30208
General Description of Cytokeratin 15.
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Human Anti-Cytokeratin 15 Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Cytokeratin 15 Antibody Clone: SPM190
Clone SPM190 Host and Isotype: Mouse IgG2a kappa
Anti-Human Cytokeratin 15 Positive Control Sample: A431 cells. Skin or Colon
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 15 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KRT15 antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 15 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the breast, respiratory epithelial cells in the bronchus and squamous epithelial cells in the esophagus. More moderate antibody staining intensity was present in glandular cells in the breast, respiratory epithelial cells in the bronchus and squamous epithelial cells in the esophagus. Low, but measureable presence of Cytokeratin 15 could be seen inglandular cells in the prostate. We were unable to detect Cytokeratin 15 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 15 expression as measured by anti-Cytokeratin 15 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - + - - - - - - - - - - - - -
KRT15 Variability + + ++ + + + ++ + ++ + + + + + + ++ + + + ++

Limitations and Warranty

enQuire Bio's Cytokeratin 15 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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