Immunohistochemical staining of Cytokeratin 18  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cytokeratin 18 [DC10] Antibody (cGMP).

$ 128.00$ 468.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD108
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD108-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD108-0.5ml Size: 0.5 ml, Format: Concentrate $ 278.00
QIVD108-1ml Size: 1 ml, Format: Concentrate $ 468.00
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SKU: QIVD108-0.1ml
Cytokeratin 18 General Information
Alternate Names
Molecular Weight
48.1 kDa
Chromosomal Location
q13.13 [chr: 12] [chr_start: 52948871] [chr_end: 52952906] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolKRT18
Entrez Gene ID3875
RefSeq Protein Accession(s)NP_000215; NP_954657
RefSeq mRNA Accession(s); NM_199187; NM_000224
RefSeq Genomic Accession(s)NC_000012; NG_008351; NG_008402
UniProt ID(s)P05783
PharmGKB ID(s)PA30217
KEGG Gene ID(s)hsa:3875
Associated Diseases (KEGG IDs)Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension. {ECO:0000269|PubMed:12724528, ECO:0000269|PubMed:9011570}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of Cytokeratin 18 .
This antibody is specific to human cytokeratin 18 of 45 kDa. Cytokeratin 18 belongs to a family of acidic type A keratins and exists along with cytokeratin 8 in most simple ductal and glandular epithelia. This antibody does not react with squamous epithelium. It reacts with benign and malignant epithelial lesions as well as a majority of adenocarcinomas and basal cell carcinomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:200
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cytokeratin 18 Antibody Clone: DC10
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Skin
Cellular Localization of Antibody DC10 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with PMC-42 human breast carcinoma cell line.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cytokeratin 18 Information for Pathologists

Summary:

Molecular weight is 45 kDa. Pairs with CK8. Ethanol causes CK8/18+ Mallory body like inclusions by (a) causing oxidative stress, which (b) inhibits proteasomes that normally remove ubiquinated cytokeratins, which (c) causes accumulation of ubiquinated cytokeratins (Exp Mol Pathol 2006;81:191). Various CK8/CK18 mutations may increase susceptibility to liver disease (Gastroenterology 2005;129:885) including cryptogenic cirrhosis (J Clin Invest 1997;99:19). Is a marker of fetal mid-face (primary palate) growth and fusion (J Dent Res 2005;84:69).

Common Uses By Pathologists:

Serum tumor marker for breast cancer (Am J Clin Pathol 2005;123:66) and T3/T4 bladder carcinoma (Clin Biochem 2002;35:327). An airway epithelial cell autoantigen associated with nonallergic asthma; a possible serum marker (also CK19) for toluene diisocyanate-induced asthma among exposed workers (Yonsei Med J 2006;47:773). RT-PCR to assess sentinel lymph nodes in colon carcinoma (Scand J Gastroenterol 2006;41:1073) and lymph nodes of gastric carcinoma (World J Gastroenterol 2005;11:6530). Loss of expression in breast carcinoma may be a poor prognostic factor (Clin Cancer Res 2004;10:2670). Cleaved cytokeratin-18 is a marker of apoptosis (J Histochem Cytochem 2005;53:229).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Cytokeratin 18 [DC10] Antibody (cGMP).