Staining by anti-Ep-CAM / CD326 Antibody 1

Anti-Epcam / CD326 Antibody Clone Epcam/1158

$ 298.00
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SKU: RT171577-MSM1
Species: Rat, Mouse, and Human (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: RT171577-MSM1-CS1
Epcam / CD326 General Information
Alternate Names
EpCAM, Epithelial cell adhesion molecule, EGP40, CD326, Cluster of Differentiation 326
Molecular Weight
40-43kDa
Chromosomal Location
6q12
Curated Database and Bioinformatic Data
Gene SymbolEpcam
Entrez Gene ID171577
Ensemble Gene IDENSRNOG00000015667
RefSeq Protein Accession(s)XP_017449516, NP_612550
RefSeq mRNA Accession(s)XM_017594027, NM_138541
RefSeq Genomic Accession(s)NC_005105, AC_000074
UniProt ID(s)O55159
UniGene ID(s)O55159
Cosmic ID(s)Epcam
KEGG Gene ID(s)rno:171577
General Description of Epcam / CD326.
Epitopes of Epcam/1158, Epcam/1159 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Epcam). It is expressed on baso-lateral cell surface in most simple epithelia, a vast majority of carcinomas.

Rat and Mouse Anti-Epcam / CD326 Antibody Product Attributes

Species: Rat and Mouse. Does not react with Human.
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Epcam / CD326 Antibody Clone: Epcam/1158
Clone Epcam/1158 Host and Isotype: Mouse IgG1
Anti-Rat, Mouse, and Human (-) Epcam / CD326 Positive Control Sample: Recombinant full-length rat Epcam protein
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: Bioreactor Concentrate with 0.05% Azide.
Antibody Concentration: Not applicable
Antibody Purification Method:Bioreactor Concentrate
Immunogen: Recombinant full-length Rat Epcam protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Epcam / CD326 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-EPCAM antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Epcam / CD326 expression as measured by anti-Epcam / CD326 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + + +++ +++ - - - +++ - - +++ - +++ - - - - +++ -
EPCAM Variability ++ +++ +++ + + + ++ + ++ + + ++ + + + ++ ++ ++ + +

Limitations and Warranty

enQuire Bio's Epcam / CD326 Anti-Rat, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Epcam / CD326 Antibody Clone Epcam/1158